Cytochrome C ELISA Kit

Produktnummer ABIN4986817

Human Cytochrome C ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Cytochrome C.

Kurzübersicht für Cytochrome C ELISA Kit (ABIN4986817)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 39-2500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Produktdetails Cytochrome C ELISA Kit

Untere Nachweisgrenze: 39 pg/mL

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Human Cytochrome C Ligand

Sensitivität: 10 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: Cytochrome C

Hintergrund: Human somatic cytochrome c is a 15 kDa, 104 amino acid (aa) polypeptide that participates in both oxidative phosphorylation and apoptosis (1). It exhibits 91 % aa identity with mouse/rat cytochrome c (1-3). The molecule is initially synthesized in the cytoplasm as an extended, 12 kDa apoprotein. This molecule is subsequently transported across the outer mitochondrial membrane into the intermitochondrial space via a receptor-independent process. Here, it associates with an inner mitochondrial membrane enzyme called CCHL (cytochrome c heme lyase) which covalently attaches one heme molecule to the N-terminus, forming functional cytochrome c (4-6). This covalent attachment causes cytochrome c to undergo a conformational change to a globular molecule effectively trapping cytochrome c in the intermitochrondrial space.Within the intermitochondrial space, cytochrome c is actively involved in the oxidative phosphorylation pathway. It transports electrons from the cytochrome c reductase complex to the cytochrome c oxidase complex (7-9). This transports excess electrons along the respiratory pathway and generates ATP for energy-dependent processes. Alternatively, and in response to apoptotic signals, cytochrome c can be released from mitochondria into the cytosol. Here, it activates an apoptotic program via one of many possible caspase-driven cascades (8, 10-12). The events which trigger an apoptotic signal (such as growth factor withdrawal) are not well understood. The result of such a signal, however, has been suggested to be a translocation of cytosolic Bax and/or Bad to the outer mitochondrial membrane where they overcome a Bcl-2-mediated stabilization (13-15). Bax-mediated destabilization may take the form of channel creation or mitochondrial swelling, resulting in cytochrome c release (15-18). Whatever the mechanism, released cytochrome c participates in the formation of a cytosolic complex which is composed of 15 kDa cytochrome c, 130 kDa Apaf-1 (apoptotic protease activating factor-1), dATP, and 46 kDa Apaf-3/caspase-9 (15, 18, 19). Within this complex, Apaf-3/caspase-9 is activated, leading to the downstream activation of caspases-3,-7 and -9, followed by additional caspases that ultimately lead to cellular apoptosis (10, 18, 20,21).

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity