IL23 ELISA Kit

Produktnummer ABIN4986949

Produktdetails IL23 ELISA Kit

Target: IL23 (Interleukin 23 (IL23))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.02-1.0 ng/mL

Untere Nachweisgrenze: 0.02 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Natural and recombinant Mouse IL-23 Ligand

Sensitivität: 7 pg/mL

Benötigtes Material:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Anwendungsinformationen

Applikationshinweise: Detection Wavelength: 450 nm

Probenmenge: 20 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL23

Target: IL23 (Interleukin 23 (IL23))

Andere Bezeichnung: IL-23 (IL23 Produkte)

Synonyme: IL-23 ELISA Kit, p19 ELISA Kit, SGRF ELISA Kit, il23p19 ELISA Kit, interleukin 23, alpha subunit p19 ELISA Kit, interleukin 23 subunit alpha ELISA Kit, Il23a ELISA Kit, IL23A ELISA Kit

Hintergrund: Interleukin 23 (IL-23) is a heterodimeric cytokine that is related to IL-12 (1-3). It is composed of two disulfide-linked subunits, a 19 kDa (p19) subunit that is unique to IL-23, and a 40 kDa (p40, IL-12) subunit that is shared with IL-12 (3-7). Mature mouse p19 and p40 share 88 % and 92 % aa sequence identity, respectively, with the corresponding rat subunits. IL-23 is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens including certain bacteria and viruses and/or their components (3, 6). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor-1 subunit (IL-12 Rβ1) and the IL-23-specific receptor subunit (IL-23 R) (7). IL-23 and IL-12 have overlapping and distinct biological activities. The IL-23 immune pathway induces the earliest recruitment of neutrophils to the site of infection, while the more classic host defense and cytotoxic response is stimulated by IL-12 (4). IL-23 has a role in the development and maintenance of a T cell subset, designated Th17, that is characterized by the production of IL-17A, IL-17F, IL-6, and TNF-α(3, 4, 8). The IL-23/IL-17 axis is an important mediator of inflammation. In mouse models, transgenic over-expression of IL-23 leads to a lethal systemic infl ammatory response (9). IL-23 eff ects on Th17 cells may also enhance the development of several models of autoimmune disease including experimental allergic encephalomyelitis (EAE), collagen-induced arthritis (CIA), colitis, and diabetes (5, 8, 10-14). IL-23 may also play a role in increased tumor growth associated with chronic inflammation (15).