CD40 Ligand ELISA Kit
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- Target Alle CD40 Ligand (CD40LG) ELISA Kits anzeigen
- CD40 Ligand (CD40LG)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 156.25-10000 pg/mL
- Untere Nachweisgrenze
- 156.25 pg/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
- Analytische Methode
- Quantitative
- Spezifität
- Natural and recombinant Human sCD40L Ligand
- Sensitivität
- 40 pg/mL
- Benötigtes Material
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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CD40 Ligand (CD40LG) ELISA Kit
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CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Maus Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Hund Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Affe Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Ratte Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Kaninchen Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reaktivität: Schwein Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- Applikationshinweise
- Detection Wavelength: 450 nm
- Probenmenge
- 20 μL
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
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- Target Alle CD40 Ligand (CD40LG) ELISA Kits anzeigen
- CD40 Ligand (CD40LG)
- Andere Bezeichnung
- sCD40L (CD40LG Produkte)
- Hintergrund
- CD40 Ligand (CD40L), also known as CD154, gp39, TNFSF5, TRAP (TNF-Related Activation Protein) or TBAM (T-cell B-cell Activating Molecule), is a multifunctional ligand in the TNF superfamily (1-4). Interaction between CD40 and CD40L is critical to the control of thymus-dependent humoral immunity and cell-mediated immune responses (5-10). The major component of the contact-dependent signal leading to B cell activation is CD40L. CD40L stimulates B cell secretion of immunoglobulin isotypes in the presence of cytokines.CD40L is a 39 kDa, 261 amino acid (aa) glycoprotein that can form homotrimers typical of other TNFSF members (1-4, 11, 12). Proteolytic cleavage can also produce 15-18 kDa soluble forms of CD40L (13, 14). Activated T cells and platelets express both a membrane-associated and a soluble form of CD40L (sCD40L) (13, 15, 16). Platelet activation during plasma and serum sample preparation can result in artificially elevated sCD40L levels (17-20). Conversely, serum samples stored above 2-8
- Pathways
- NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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