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Thymic Stromal Lymphopoietin ELISA Kit

TSLP Reaktivität: Maus Colorimetric Sandwich ELISA 0.02-1.0 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Produktnummer ABIN4987142
  • Target Alle Thymic Stromal Lymphopoietin (TSLP) ELISA Kits anzeigen
    Thymic Stromal Lymphopoietin (TSLP)
    Reaktivität
    • 13
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    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.02-1.0 ng/mL
    Untere Nachweisgrenze
    0.02 ng/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Natural and recombinant Mouse TSLP Ligand
    Sensitivität
    7 pg/mL
    Benötigtes Material
    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
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  • Applikationshinweise
    Detection Wavelength: 450 nm
    Probenmenge
    20 μL
    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle Thymic Stromal Lymphopoietin (TSLP) ELISA Kits anzeigen
    Thymic Stromal Lymphopoietin (TSLP)
    Andere Bezeichnung
    TSLP (TSLP Produkte)
    Hintergrund
    Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell-stimulating factor family. Originally identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development, TSLP is expressed in thymus, spleen, kidney, lung, and bone marrow. Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. TSLP activity overlaps with that of IL-7, playing a key role in the development of B cells, as well as stimulating the growth of thymocytes and T cells. TSLP stimulates monocytes to produce T cell-attracting chemokines such as TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines, including IL-4, IL-5, IL-13, and TNFα. This hematopoietic cytokine binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Binding induces activation of STAT3 and STAT5 via phosphorylation. Mouse TSLP shares approximately 43 % amino acid sequence identity with human TSLP.
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