MIF ELISA Kit

Produktnummer ABIN5026922

Produktdetails MIF ELISA Kit

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781-50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human MIF concentrations in serum, plasma and other biological fluids.

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: < 0.469 ng/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Andere Bezeichnung: MIF (Macrophage migration inhibitory factor) (MIF Produkte)

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling