Melatonin Sulfate ELISA Kit
Kurzübersicht für Melatonin Sulfate ELISA Kit (ABIN5067888)
Target
Alle Melatonin Sulfate (MS) ProdukteReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- Enzyme immunoassay for the quantitative determination of melatonin sulfate in human urine.
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Analytische Methode
- Quantitative
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Sensitivität
- 1 ng/mL
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Produktmerkmale
- Melatonin Sulfate, Human (6-Hydroxymelatonin Sulfate, 6-Sulfatoxymelatonin) ELISA Kit
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Bestandteile
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- Microtiter Plate, 1x96 wells
- PAB, 1x200ul
- Melatonin Sulfate (HRP), 40x, 1x200ul
- Standard, 0ng/ml, 1x100ul
- Standard, 1.7ng/ml, 1x100ul
- Standard, 5.2ng/ml, 1x100ul
- Standard, 15.6ng/ml, 1x100ul
- Standard, 46.7ng/ml, 1x100ul
- Standard, 140ng/ml, 1x100ul
- 0: Standard, 420ng/ml, 1x100ul
- 1: Low Control 1, 1x100ul
- 2: High Control 2, 1x100ul
- 3: Assay Buffer, 1x60ml
- 4: Wash Buffer, 20x, 1x50ml
- 5: TMB Substrate, 1x12ml
- 6: Stop Solution, 1M O4, 1x12ml
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Protokoll
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Principle:
- Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 4°C
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- Melatonin Sulfate (MS)
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Andere Bezeichnung
- Melatonin Sulfate
Target Alle Melatonin Sulfate (MS) Produkte
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