Phenylalanine Hydroxylase ELISA Kit

Produktnummer ABIN5067907

Produktdetails Phenylalanine Hydroxylase ELISA Kit

Target: Phenylalanine Hydroxylase

Reaktivität: Chemical

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: PAH (Phenylalanine Hydroxylase) ELISA Kit is a quantitative sandwich assay for the detection of PAH in human serum, plasma, tissue homogenates and other biological fluids

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 46.875 pg/mL

Produktmerkmale: PAH (Phenylalanine Hydroxylase) ELISA Kit (Human)

Bestandteile:

• *357883A: Microtiter Strips, 1x96 wells (8x12 wells).
• *357883B: Standard, 2x1 vial
• 357883C: Sample/Standard Dilution Buffer, 1x20ml
• 357883D: Antibody (Biotin) (Concentrated), 1x120ul
• 357883E: Antibody Dilution Buffer, 1x10ml
• 357883F: Streptavidin (HRP) (SABC), 1x120ul
• 357883G: SABC Dilution Buffer, 1x10ml
• 357883H: TMB Substrate, 1x10ml
• 357883J: Stop Solution, 1x10ml
• 357883K: Wash Buffer, 25X, 1x30ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to PAH. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain PAH, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAH in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Testpräzision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu Phenylalanine Hydroxylase

Target: Phenylalanine Hydroxylase

Andere Bezeichnung: PAH (Phenylalanine Hydroxylase) (Phenylalanine Hydroxylase Produkte)

Substanzklasse: Chemical