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Mesothelin ELISA Kit

MSLN Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Lysate, Plasma, Serum
Produktnummer ABIN5068269
  • Target Alle Mesothelin (MSLN) ELISA Kits anzeigen
    Mesothelin (MSLN)
    Reaktivität
    • 9
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Proben
    Cell Culture Lysate, Plasma, Serum
    Analytische Methode
    Quantitative
    Sensitivität
    0.010 ng/mL
    Produktmerkmale
    Mesothelin, ELISA Kit (Human) (CAK1 Antigen, Pre-pro-megakaryocyte-potentiating Factor, MSLN, MPF)
    Bestandteile
    • Mesothelin Microplate: 12 strips x 8 wells
    • Mesothelin monoclonal antibody, conjugated to HRP: 1 x 21ml
    • Mesothelin Standard (lyophilized): 1 x 100ng
    • Assay Diluent R 1 x 11ml
    • Calibrator Diluent R Concentrate: 1 x 21ml
    • Wash Buffer Concentrate: 1 x 21ml
    • Color Reagent A (stabilized hydrogen peroxide): 1 x 12.5ml
    • Color Reagent B (tetramethylbenzidine): 1 x 12.5ml
    • Stop Solution (2N sulfuric acid): 1 x 6ml
    • Plate Covers: 4 adhesive strips
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  • Plattentyp
    Pre-coated
    Protokoll
    Principle:
    • This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mesothelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Mesothelin present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for Mesothelin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Mesothelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Informationen zur Lagerung
    4°C
  • Target Alle Mesothelin (MSLN) ELISA Kits anzeigen
    Mesothelin (MSLN)
    Andere Bezeichnung
    Mesothelin (MSLN Produkte)
    Synonyme
    MPF ELISA Kit, SMRP ELISA Kit, MSLN ELISA Kit, mesothelin ELISA Kit, MSLN ELISA Kit, Msln ELISA Kit, LOC611363 ELISA Kit, LOC100524016 ELISA Kit
    Hintergrund
    Mesothelin, also known as CAK1 and ERC, is a protein that is mainly expressed in the mesothelial cells lining the pleura, pericardium, and peritoneum. The Mesothelin gene encodes a precursor protein of 70kD that is cleaved into two products, including megakaryocyte potentiating factor (aa37-286) and Mesothelin (aa296-606). Megakaryocyte potentiation factor is a secreted protein of about 32kD, and it functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes.
    Pathways
    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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