Free Thyroxine ELISA Kit

Produktnummer ABIN5068280

Produktdetails Free Thyroxine ELISA Kit

Target: Free Thyroxine (fT4) (Free Thyroxine T4 (fT4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Plasma, Cell Culture Supernatant, Serum, Tissue Samples

Sensitivität: 2 pg/mL

Produktmerkmale: Free Thyroxine (fT4) ELISA Kit (Human) (Thyroxine, FT4)

Bestandteile:

• Aluminium pouches with a Microwell Plate: 8x12
• Standard: 2xVial
• Biotinylated antibody: 2xVial
• Streptavidin-HRP: 2xVial
• Assay Buffer (25ml/Vial): 2xVial
• Wash Buffer Concentrate 20x (30ml/Vial): 1xVial
• TMB Substrate Solution: 1x12ml
• Stop Solution (12ml/Vial): 1xVial
• Plate Covers- Adhesive strips: 3xstrips
• Instruction: 1x

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• This assay employs the Sandwich immunoassay technique. An anti-h FT4 monoclonal coating antibody is adsorbed onto microwells. FT4 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h FT4 antibody is added and binds to FT4 captured by the first antibody. Following incubation unbound Biotinylated anti-h FT4 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h FT4 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of FT4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven FT4 standard dilutions and FT4 sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu Free Thyroxine

Target: Free Thyroxine (fT4) (Free Thyroxine T4 (fT4))

Andere Bezeichnung: Free Thyroxine (fT4) (fT4 Produkte)

Hintergrund: Thyroxine (T4) is a tyrosine-based hormone produced by the thyroid glands. Thyroxine circulates throughout the body primarily bound to carrier proteins. Free T4 is converted to Triiodothyronine (T3) in peripheral tissues. The thyro-nines increase the basal metabolic rate, affect protein synthesis and increase the sensitivity of the body to catecholamines (such as adrenaline). Cases of hypothyroidism, where the gland is insufficiently active, can be treated by administration of Thyroxine or a combination of Thyroxine and triiodothyronine. Sufficient levels of maternal Thyroxine is essential for fetal development, and inadequate production can cause irreversible fetal brain damage.