DDR1 ELISA Kit

Produktnummer ABIN5526735

Produktdetails DDR1 ELISA Kit

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Bindungsspezifität: pTyr792, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit. This assay semi-quantitatively measures DDR1 phosphorylated at Tyrosine-792 as well as total DDR1 in cell lysate samples.

Proben: Cell Culture Lysate

Analytische Methode: Semi-Quantitative

Spezifität: This ELISA kit recognizes Human DDR1 phosphorylated at site Tyrosine-792 as well as total DDR1.

Produktmerkmale:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu DDR1

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Andere Bezeichnung: DDR1 (DDR1 Produkte)

Synonyme: CG11573 ELISA Kit, CG13984 ELISA Kit, CG13985 ELISA Kit, CG33531 ELISA Kit, CG9488 ELISA Kit, CG9490 ELISA Kit, CT33540 ELISA Kit, DDR ELISA Kit, Dmel\\CG33531 ELISA Kit, Dmel_CG13984 ELISA Kit, 6030432F18 ELISA Kit, AI323681 ELISA Kit, CD167a ELISA Kit, Cak ELISA Kit, Nep ELISA Kit, PTK3A ELISA Kit, CAK ELISA Kit, CD167 ELISA Kit, EDDR1 ELISA Kit, HGK2 ELISA Kit, MCK10 ELISA Kit, NEP ELISA Kit, NTRK4 ELISA Kit, PTK3 ELISA Kit, RTK6 ELISA Kit, TRKE ELISA Kit, Drd1 ELISA Kit, PTK3D ELISA Kit, Discoidin domain receptor ELISA Kit, discoidin domain receptor family, member 1 ELISA Kit, discoidin domain receptor tyrosine kinase 1 ELISA Kit, Ddr ELISA Kit, Ddr1 ELISA Kit, DDR1 ELISA Kit

Gen-ID: 780

UniProt: Q08345

Pathways: RTK Signalweg, Smooth Muscle Cell Migration