Exosome Purification and Analysis Kit (Cell culture)
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- Reaktivität
- Human
- Nachweismethode
- Fluorometric
- Konjugat
- PE
- Applikation
- Flow Cytometry (FACS)
- Verwendungszweck
- Exosome purification and FACS analysis kit of exosomes derived from cell culture
- Marke
- ExoStep™
- Proben
- Cell Culture Cells
- Produktmerkmale
- The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
- Bestandteile
- CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X
- Benötigtes Material
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• Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer
- tubes
). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume
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Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Applikationshinweise
- Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes
- Kommentare
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Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol
- Probenmenge
- 50 μL
- Testdauer
- 4 h
- Aufbereitung der Reagenzien
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Reagents ready to use.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store in the dark at 2-8 °C
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- Hintergrund
- Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
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