DHCR7 ELISA Kit

Produktnummer ABIN5651296

Produktdetails DHCR7 ELISA Kit

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of 7-Dehydrocholesterol Reductase (DHCR7). No significant cross-reactivity or interference between 7-Dehydrocholesterol Reductase (DHCR7) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 7-Dehydrocholesterol Reductase (DHCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 7-Dehydrocholesterol Reductase (DHCR7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu DHCR7

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Andere Bezeichnung: 7-Dehydrocholesterol Reductase (DHCR7 Produkte)

Synonyme: 7-Dhcr ELISA Kit, Xdhcr7 ELISA Kit, MGC64361 ELISA Kit, slos ELISA Kit, 7-STEROL DELTA7 REDUCTASE ELISA Kit, 7RED ELISA Kit, DELTA5 ELISA Kit, DWARF 5 ELISA Kit, F11F12.21 ELISA Kit, F11F12_21 ELISA Kit, LE ELISA Kit, LEPIDA ELISA Kit, PA ELISA Kit, PARVA ELISA Kit, ST7R ELISA Kit, STEROL DELTA7 REDUCTASE ELISA Kit, DKFZp468C1911 ELISA Kit, AA409147 ELISA Kit, SLOS ELISA Kit, xdhcr7 ELISA Kit, 7-dehydrocholesterol reductase ELISA Kit, 7-dehydrocholesterol reductase L homeolog ELISA Kit, Ergosterol biosynthesis ERG4/ERG24 family ELISA Kit, DHCR7 ELISA Kit, dhcr7 ELISA Kit, dhcr7.L ELISA Kit, DWF5 ELISA Kit, PAAG_04766 ELISA Kit, PITG_13128 ELISA Kit, VDBG_05423 ELISA Kit, MGYG_00712 ELISA Kit, TERG_00580 ELISA Kit, Dhcr7 ELISA Kit

Hintergrund:

Gene Name: 7-Dehydrocholesterol Reductase

Gene Aliases: DHCR-7, SLOS, D7SR, Smith-Lemli-Opitz Syndrome, Putative sterol reductase SR-2, Sterol Delta(7)-reductase

Gen-ID: 1717

UniProt: Q9UBM7