ADP ELISA Kit

Produktnummer ABIN5651421

Produktdetails ADP ELISA Kit

Target: ADP (Adenosine Diphosphate (ADP))

Reaktivität: Diverse Spezies

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 18.52 ng/mL - 1500 ng/mL

Untere Nachweisgrenze: 18.52 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Adenosine Diphosphate (ADP). No significant cross-reactivity or interference between Adenosine Diphosphate (ADP) and analogues was observed.

Sensitivität: 7.25 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenosine Diphosphate (ADP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenosine Diphosphate (ADP) and unlabeled Adenosine Diphosphate (ADP) (Standards or samples) with the pre-coated antibody specific to Adenosine Diphosphate (ADP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenosine Diphosphate (ADP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenosine Diphosphate (ADP) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosine Diphosphate (ADP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosine Diphosphate (ADP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ADP

Target: ADP (Adenosine Diphosphate (ADP))

Andere Bezeichnung: Adenosine Diphosphate (ADP Produkte)

Synonyme: 6720489L24Rik ELISA Kit, 9330209L04Rik ELISA Kit, Adp ELISA Kit, Mamdc1 ELISA Kit, RNSVSP4U ELISA Kit, SVSIV1 ELISA Kit, Svp4 ELISA Kit, ADP ELISA Kit, DCAF9 ELISA Kit, MAM domain containing glycosylphosphatidylinositol anchor 2 ELISA Kit, seminal vesicle secretory protein 4 ELISA Kit, WD and tetratricopeptide repeats 1 ELISA Kit, Mdga2 ELISA Kit, Svs4 ELISA Kit, WDTC1 ELISA Kit

Hintergrund:

Gene Name: Adenosine Diphosphate

Pathways: Regulation of Cell Size