GRK2 ELISA Kit

Produktnummer ABIN5651480

Produktdetails GRK2 ELISA Kit

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Beta Kinase 1 (ADRbK1). No significant cross-reactivity or interference between Adrenergic Receptor Beta Kinase 1 (ADRbK1) and analogues was observed.

Sensitivität: 0.125 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Beta Kinase 1 (ADRbK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Beta Kinase 1 (ADRbK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Beta Kinase 1 (ADRbK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Beta Kinase 1 (ADRbK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Beta Kinase 1 (ADRbK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Beta Kinase 1 (ADRbK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Andere Bezeichnung: Adrenergic Receptor Beta Kinase 1 (ADRBK1 Produkte)

Synonyme: Adrbk-1 ELISA Kit, Bark-1 ELISA Kit, GRK2 ELISA Kit, betaARK1 ELISA Kit, BARK1 ELISA Kit, BETA-ARK1 ELISA Kit, GRK-2 ELISA Kit, Grk2 ELISA Kit, beta-adrenergic receptor kinase 1 ELISA Kit, G protein-coupled receptor kinase 2 ELISA Kit, Tsp_00497 ELISA Kit, grk2 ELISA Kit, Grk2 ELISA Kit, GRK2 ELISA Kit

Hintergrund:

Gene Name: Adrenergic Receptor Beta Kinase 1

Gene Aliases: B-ARK1, bARK1, Beta-ARK1, GRK2, G-protein coupled receptor kinase 2

Gen-ID: 156

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling