ALDH3A1 ELISA Kit

Produktnummer ABIN5651559

Produktdetails ALDH3A1 ELISA Kit

Target: ALDH3A1 (Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1). No significant cross-reactivity or interference between Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) and analogues was observed.

Sensitivität: 0.32 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ALDH3A1

Target: ALDH3A1 (Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1))

Andere Bezeichnung: Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1 Produkte)

Synonyme: ALDC ELISA Kit, ALDH-E1 ELISA Kit, ALDH1 ELISA Kit, ALDH11 ELISA Kit, PUMB1 ELISA Kit, RALDH1 ELISA Kit, ALDH3 ELISA Kit, ALDHIII ELISA Kit, Ahd-4 ELISA Kit, Ahd4 ELISA Kit, Aldh ELISA Kit, Aldh3 ELISA Kit, AHDC ELISA Kit, Ahd-c ELISA Kit, aldehyde dehydrogenase 1 family member A1 ELISA Kit, aldehyde dehydrogenase 3 family member A1 ELISA Kit, aldehyde dehydrogenase ELISA Kit, aldehyde dehydrogenase, dimeric NADP-preferring ELISA Kit, aldehyde dehydrogenase family 3, subfamily A1 ELISA Kit, aldehyde dehydrogenase 3 family, member A1 ELISA Kit, ALDH1A1 ELISA Kit, ALDH3A1 ELISA Kit, OE_RS03630 ELISA Kit, LOC100077088 ELISA Kit, CLL_A1665 ELISA Kit, LOC100281700 ELISA Kit, LOC100285809 ELISA Kit, Aldh3a1 ELISA Kit

Hintergrund:

Gene Name: Aldehyde Dehydrogenase 3 Family, Member A1

Gene Aliases: ALDH3, ALDHIII, Aldehyde Dehydrogenase,Dimeric NADP-Preferring, Aldehyde dehydrogenase 3

Gen-ID: 216

UniProt: P00352