AADAT ELISA Kit

Produktnummer ABIN5651671

Produktdetails AADAT ELISA Kit

Target: AADAT (Aminoadipate Aminotransferase (AADAT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Aminoadipate Aminotransferase (AADAT). No significant cross-reactivity or interference between Aminoadipate Aminotransferase (AADAT) and analogues was observed.

Sensitivität: 0.067 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoadipate Aminotransferase (AADAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoadipate Aminotransferase (AADAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoadipate Aminotransferase (AADAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminoadipate Aminotransferase (AADAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminoadipate Aminotransferase (AADAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminoadipate Aminotransferase (AADAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu AADAT

Target: AADAT (Aminoadipate Aminotransferase (AADAT))

Andere Bezeichnung: Aminoadipate Aminotransferase (AADAT Produkte)

Synonyme: KAT2 ELISA Kit, KATII ELISA Kit, AI875679 ELISA Kit, Aadt ELISA Kit, Kat2 ELISA Kit, mKat-2 ELISA Kit, MGC80030 ELISA Kit, kat2 ELISA Kit, katii ELISA Kit, aminoadipate aminotransferase ELISA Kit, aminoadipate aminotransferase S homeolog ELISA Kit, AADAT ELISA Kit, Aadat ELISA Kit, aadat.S ELISA Kit, aadat ELISA Kit

Hintergrund:

Gene Name: Aminoadipate Aminotransferase

Gene Aliases: KATII, KAT2, Kynurenine Aminotransferase II, L Kynurenine/Alpha Aminoadipate Aminotransferase, Kynurenine aminotransferase II

Gen-ID: 51166

UniProt: Q8N5Z0