AGR2 ELISA Kit

Produktnummer ABIN5651847

Produktdetails AGR2 ELISA Kit

Target: AGR2 (Anterior Gradient Homolog 2 (Xenopus Laevis) (AGR2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Anterior Gradient Protein 2 (AGR2). No significant cross-reactivity or interference between Anterior Gradient Protein 2 (AGR2) and analogues was observed.

Sensitivität: 0.62 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient Protein 2 (AGR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anterior Gradient Protein 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anterior Gradient Protein 2 (AGR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anterior Gradient Protein 2 (AGR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anterior Gradient Protein 2 (AGR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anterior Gradient Protein 2 (AGR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu AGR2

Target: AGR2 (Anterior Gradient Homolog 2 (Xenopus Laevis) (AGR2))

Andere Bezeichnung: Anterior Gradient Protein 2 (AGR2 Produkte)

Synonyme: AG2 ELISA Kit, GOB-4 ELISA Kit, HAG-2 ELISA Kit, PDIA17 ELISA Kit, XAG-2 ELISA Kit, Agr2h ELISA Kit, Gob-4 ELISA Kit, mAG-2 ELISA Kit, wu:fj29g05 ELISA Kit, zgc:112187 ELISA Kit, agr2 ELISA Kit, hag3 ELISA Kit, hag-3 ELISA Kit, bcmp11 ELISA Kit, MGC89004 ELISA Kit, XAgr2 ELISA Kit, agr2-A ELISA Kit, anterior gradient 2, protein disulphide isomerase family member ELISA Kit, anterior gradient 2 ELISA Kit, anterior gradient 3, protein disulphide isomerase family member ELISA Kit, anterior gradient 2, protein disulphide isomerase family member S homeolog ELISA Kit, AGR2 ELISA Kit, Agr2 ELISA Kit, agr2 ELISA Kit, agr3 ELISA Kit, agr2.S ELISA Kit

Hintergrund:

Gene Name: Anterior Gradient Protein 2

Gene Aliases: GOB4, HAG2, XAG2, AG2, PDIA17, HPC8, Protein Disulfide Isomerase Family A,Member 17, Secreted cement gland protein XAG-2 homolog

Gen-ID: 10551

UniProt: O95994