APOO ELISA Kit

Produktnummer ABIN5651954

Produktdetails APOO ELISA Kit

Target: APOO (Apolipoprotein O (APOO))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein O (APOO). No significant cross-reactivity or interference between Apolipoprotein O (APOO) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein O (APOO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein O (APOO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein O (APOO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein O (APOO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein O (APOO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein O (APOO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu APOO

Target: APOO (Apolipoprotein O (APOO))

Andere Bezeichnung: Apolipoprotein O (APOO Produkte)

Synonyme: fam121b ELISA Kit, fp74f12 ELISA Kit, wu:fp74f12 ELISA Kit, zgc:123314 ELISA Kit, my025 ELISA Kit, FAM121B ELISA Kit, 0610008C08Rik ELISA Kit, 1110019O03Rik ELISA Kit, RGD1565289 ELISA Kit, MGC79016 ELISA Kit, si:rp71-1f1.3 ELISA Kit, wu:fr42a11 ELISA Kit, zgc:103766 ELISA Kit, apolipoprotein O, a ELISA Kit, apolipoprotein O ELISA Kit, apolipoprotein O L homeolog ELISA Kit, apolipoprotein O, b ELISA Kit, apooa ELISA Kit, apoo ELISA Kit, APOO ELISA Kit, Apoo ELISA Kit, apoo.L ELISA Kit, apoob ELISA Kit

Hintergrund:

Gene Name: Apolipoprotein O

Gene Aliases: Apo-O, FAM121B, My025, Family With Sequence Similarity 121B

Gen-ID: 79135

UniProt: Q9BUR5