Asparagine Synthetase ELISA Kit

Produktnummer ABIN5652041

Produktdetails Asparagine Synthetase ELISA Kit

Target: Asparagine Synthetase (ASNS)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Asparagine Synthetase (ASNS). No significant cross-reactivity or interference between Asparagine Synthetase (ASNS) and analogues was observed.

Sensitivität: 0.108 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asparagine Synthetase (ASNS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asparagine Synthetase (ASNS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asparagine Synthetase (ASNS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Asparagine Synthetase (ASNS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Asparagine Synthetase

Target: Asparagine Synthetase (ASNS)

Andere Bezeichnung: Asparagine Synthetase (ASNS Produkte)

Synonyme: zgc:56605 ELISA Kit, zgc:76901 ELISA Kit, LOC692995 ELISA Kit, ASNS ELISA Kit, GB13219 ELISA Kit, CG33486 ELISA Kit, Dmel\\CG33486 ELISA Kit, asns ELISA Kit, TS11 ELISA Kit, ARABIDOPSIS THALIANA GLUTAMINE-DEPENDENT ASPARAGINE SYNTHASE 1 ELISA Kit, AT-ASN1 ELISA Kit, DARK INDUCIBLE 6 ELISA Kit, DIN6 ELISA Kit, GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE ELISA Kit, glutamine-dependent asparagine synthase 1 ELISA Kit, AS ELISA Kit, AsnS1 ELISA Kit, asparagine synthetase ELISA Kit, asparagine synthetase (glutamine-hydrolyzing) ELISA Kit, asparagine synthetase [glutamine-hydrolyzing] ELISA Kit, Asparagine synthetase ELISA Kit, glutamine-dependent asparagine synthase 1 ELISA Kit, asparagine synthetase homolog 1 ELISA Kit, asns ELISA Kit, ASNS ELISA Kit, LOC692995 ELISA Kit, LOC724480 ELISA Kit, asnB ELISA Kit, AsnS ELISA Kit, Asns ELISA Kit, ASN1 ELISA Kit, asn1 ELISA Kit

Hintergrund:

Gene Name: Asparagine Synthetase

Gene Aliases: Aspartate-Ammonia Ligase, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase

Gen-ID: 27053

UniProt: Q61024

Pathways: ER-Nucleus Signaling