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BNIP3 ELISA Kit

BNIP3 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5652171
  • Target Alle BNIP3 ELISA Kits anzeigen
    BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
    Reaktivität
    • 2
    • 2
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.625 ng/mL - 40 ng/mL
    Untere Nachweisgrenze
    0.625 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). No significant cross-reactivity or interference between Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3) and analogues was observed.
    Sensitivität
    0.228 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3 (BNIP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle BNIP3 ELISA Kits anzeigen
    BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
    Andere Bezeichnung
    Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3 Produkte)
    Synonyme
    NIP3 ELISA Kit, Nip3 ELISA Kit, BCL2 interacting protein 3 ELISA Kit, BCL2/adenovirus E1B interacting protein 3 ELISA Kit, BNIP3 ELISA Kit, Bnip3 ELISA Kit
    Hintergrund

    Gene Name: Bcl2/Adenovirus E1B 19 kDa Interacting Protein 3

    Gene Aliases: NIP3

    Gen-ID
    84480
    UniProt
    Q9ET45
    Pathways
    Autophagie, Brown Fat Cell Differentiation
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