BLMH CLIA Kit

Produktnummer ABIN5652233

Produktdetails BLMH CLIA Kit

Target: BLMH (Bleomycin Hydrolase (BLMH))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Bleomycin Hydrolase (BLMH). No significant cross-reactivity or interference between Bleomycin Hydrolase (BLMH) and analogues was observed.

Sensitivität: 5.04 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Bleomycin Hydrolase (BLMH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bleomycin Hydrolase (BLMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bleomycin Hydrolase (BLMH) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: BLMH (Bleomycin Hydrolase (BLMH))

Andere Bezeichnung: Bleomycin Hydrolase (BLMH Produkte)

Synonyme: AI035728 CLIA Kit, Bh CLIA Kit, Bmh CLIA Kit, wu:fb13c01 CLIA Kit, zgc:66261 CLIA Kit, bh CLIA Kit, bmh CLIA Kit, BH CLIA Kit, BMH CLIA Kit, bleomycin hydrolase CLIA Kit, Bleomycin hydrolase CLIA Kit, bleomycin hydrolase S homeolog CLIA Kit, BLMH CLIA Kit, pepC CLIA Kit, LOC5569945 CLIA Kit, Apre_0140 CLIA Kit, Apar_0899 CLIA Kit, Smon_1289 CLIA Kit, Kfla_1242 CLIA Kit, Snas_3912 CLIA Kit, BLJ_1941 CLIA Kit, Olsu_1085 CLIA Kit, PGTDC60_0106 CLIA Kit, Blmh CLIA Kit, blmh CLIA Kit, blmh.S CLIA Kit

Hintergrund:

Gene Name: Bleomycin Hydrolase

Gene Aliases: BH, BMH

Gen-ID: 642

UniProt: Q13867