BDKRB2 ELISA Kit

Produktnummer ABIN5652310

Produktdetails BDKRB2 ELISA Kit

Target: BDKRB2 (Bradykinin Receptor B2 (BDKRB2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Bradykinin Receptor B2 (BDKRB2). No significant cross-reactivity or interference between Bradykinin Receptor B2 (BDKRB2) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bradykinin Receptor B2 (BDKRB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bradykinin Receptor B2 (BDKRB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bradykinin Receptor B2 (BDKRB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bradykinin Receptor B2 (BDKRB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bradykinin Receptor B2 (BDKRB2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bradykinin Receptor B2 (BDKRB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu BDKRB2

Target: BDKRB2 (Bradykinin Receptor B2 (BDKRB2))

Andere Bezeichnung: Bradykinin Receptor B2 (BDKRB2 Produkte)

Synonyme: BDKRB2 ELISA Kit, b2r ELISA Kit, bk2 ELISA Kit, bk-2 ELISA Kit, bkr2 ELISA Kit, brb2 ELISA Kit, kinrec ELISA Kit, B2R ELISA Kit, BK-2 ELISA Kit, BK2 ELISA Kit, BKR2 ELISA Kit, BRB2 ELISA Kit, B2BKR ELISA Kit, B2BRA ELISA Kit, B(2) ELISA Kit, B2 ELISA Kit, BK2R ELISA Kit, Bdkrb2 ELISA Kit, bradykinin receptor B2 ELISA Kit, bradykinin receptor, beta 2 ELISA Kit, bradykinin type 2 receptor ELISA Kit, Bdkrb2 ELISA Kit, BDKRB2 ELISA Kit, bdkrb2 ELISA Kit, kinrec ELISA Kit, B2R ELISA Kit

Hintergrund:

Gene Name: Bradykinin Receptor B2

Gene Aliases: BDKR-B2, B2R, BK2, BKR2, BRB2

Pathways: ACE Inhibitor Pathway, Negative Regulation of intrinsic apoptotic Signaling