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LI Cadherin ELISA Kit

Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5652448
  • Target Alle LI Cadherin Produkte
    LI Cadherin
    Reaktivität
    • 3
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.25 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.25 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cadherin 17 (CDH17). No significant cross-reactivity or interference between Cadherin 17 (CDH17) and analogues was observed.
    Sensitivität
    11.6 pg/mL
    Featured
    Zu unserem meistverkauften LI Cadherin ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 17 (CDH17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 17 (CDH17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 17 (CDH17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin 17 (CDH17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin 17 (CDH17) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin 17 (CDH17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle LI Cadherin Produkte
    LI Cadherin
    Andere Bezeichnung
    Cadherin 17 (LI Cadherin Produkte)
    Synonyme
    CDH16 ELISA Kit, HPT-1 ELISA Kit, HPT1 ELISA Kit, HPT-1/LI ELISA Kit, cb903 ELISA Kit, fb65d02 ELISA Kit, wu:fb65d02 ELISA Kit, cadherin-17 ELISA Kit, cadherin 17 ELISA Kit, cadherin 17, LI cadherin (liver-intestine) ELISA Kit, CDH17 ELISA Kit, Cdh17 ELISA Kit, cdh17 ELISA Kit
    Hintergrund

    Gene Name: Cadherin 17

    Gene Aliases: CDH16, HPT-1, HPT1, LI-Cadherin, Cadherin,Liver Intestine, Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin

    Gen-ID
    1015
    UniProt
    Q12864
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