Calpain 3 ELISA Kit

Produktnummer ABIN5652515

Produktdetails Calpain 3 ELISA Kit

Target: Calpain 3 (CAPN3)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Calpain 3 (CAPN3). No significant cross-reactivity or interference between Calpain 3 (CAPN3) and analogues was observed.

Sensitivität: 0.125 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 3 (CAPN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 3 (CAPN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 3 (CAPN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain 3 (CAPN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain 3 (CAPN3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain 3 (CAPN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Calpain 3

Target: Calpain 3 (CAPN3)

Andere Bezeichnung: Calpain 3 (CAPN3 Produkte)

Synonyme: capn3 ELISA Kit, MGC80925 ELISA Kit, zgc:92687 ELISA Kit, CAPN3 ELISA Kit, AI323605 ELISA Kit, Capa-3 ELISA Kit, Capa3 ELISA Kit, Lp82 ELISA Kit, p94 ELISA Kit, Lp84 ELISA Kit, Lp85 ELISA Kit, CANP3 ELISA Kit, CANPL3 ELISA Kit, LGMD2 ELISA Kit, LGMD2A ELISA Kit, nCL-1 ELISA Kit, SKM-CALP ELISA Kit, SKM-CALPOV ELISA Kit, nCL1 ELISA Kit, calpain 1, (mu/I) large subunit L homeolog ELISA Kit, calpain 3a, (p94) ELISA Kit, calpain 3 ELISA Kit, capn1.L ELISA Kit, capn3a ELISA Kit, CAPN3 ELISA Kit, Capn3 ELISA Kit

Hintergrund:

Gene Name: Calpain 3

Gene Aliases: CANPL3, p94, LGMD2, LGMD2A, nCL1, Calcium-activated neutral proteinase 3, Muscle-specific calcium-activated neutral protease 3, New calpain 1

Gen-ID: 825

UniProt: P20807

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development