CEACAM6 ELISA Kit

Produktnummer ABIN5652591

Produktdetails CEACAM6 ELISA Kit

Target: CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 23.44 pg/mL - 1500 pg/mL

Untere Nachweisgrenze: 23.44 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6). No significant cross-reactivity or interference between Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) and analogues was observed.

Sensitivität: 9.68 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CEACAM6

Target: CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6))

Andere Bezeichnung: Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6 Produkte)

Synonyme: CD66c ELISA Kit, CEAL ELISA Kit, NCA ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 6 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 6 ELISA Kit, Ceacam6 ELISA Kit, CEACAM6 ELISA Kit

Hintergrund:

Gene Name: Carcinoembryonic Antigen Related Cell Adhesion Molecule 6

Gene Aliases: CD66c, NCA, CD66-C, CEAL, Non-Specific Cross Reacting Antigen, Normal cross-reacting antigen

Gen-ID: 4680

UniProt: P40199