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Casein alpha S1 ELISA Kit

CSN1S1 Reaktivität: Rind (Kuh) Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Milk
Produktnummer ABIN5652632
  • Target Alle Casein alpha S1 (CSN1S1) ELISA Kits anzeigen
    Casein alpha S1 (CSN1S1)
    Reaktivität
    • 4
    • 3
    • 1
    • 1
    • 1
    Rind (Kuh)
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.25 ng/mL - 2000 ng/mL
    Untere Nachweisgrenze
    31.25 ng/mL
    Applikation
    ELISA
    Proben
    Milk
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Casein Alpha (CSN1). No significant cross-reactivity or interference between Casein Alpha (CSN1) and analogues was observed.
    Sensitivität
    13.8 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Alpha (CSN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Alpha (CSN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Alpha (CSN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Alpha (CSN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Alpha (CSN1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Alpha (CSN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Casein alpha S1 (CSN1S1) ELISA Kits anzeigen
    Casein alpha S1 (CSN1S1)
    Andere Bezeichnung
    Casein Alpha (CSN1S1 Produkte)
    Synonyme
    CSN1S1 ELISA Kit, CASA ELISA Kit, CSN1 ELISA Kit, AA959832 ELISA Kit, AW212882 ELISA Kit, Csna ELISA Kit, Casa ELISA Kit, Csn1 ELISA Kit, CSCN1 ELISA Kit, casein alpha s1 ELISA Kit, CSN1S1 ELISA Kit, Csn1s1 ELISA Kit
    Hintergrund

    Gene Name: Casein Alpha

    Gene Aliases: CASa, CSN1, CSN1S1, Casein Alpha S1, Caseinate, Caseine/caséine/Kasein/caseína, Alpha-S1-casein, Casoxin-D

    Gen-ID
    282208
    UniProt
    P02662
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