CTNNA1 ELISA Kit

Produktnummer ABIN5652678

Produktdetails CTNNA1 ELISA Kit

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Catenin Alpha 1 (CTNNa1). No significant cross-reactivity or interference between Catenin Alpha 1 (CTNNa1) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Alpha 1 (CTNNa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Alpha 1 (CTNNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Alpha 1 (CTNNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Alpha 1 (CTNNa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Andere Bezeichnung: Catenin Alpha 1 (CTNNA1 Produkte)

Synonyme: CAP102 ELISA Kit, 2010010M04Rik ELISA Kit, AA517462 ELISA Kit, AI988031 ELISA Kit, Catna1 ELISA Kit, CG17947 ELISA Kit, CT39986 ELISA Kit, Dalpha-Cat ELISA Kit, Dalpha-cat ELISA Kit, DalphaC ELISA Kit, Dalphacat ELISA Kit, Dmcatp ELISA Kit, Dmel\\CG17947 ELISA Kit, a-Cat ELISA Kit, a-cat ELISA Kit, alpha-CAT ELISA Kit, alpha-cat ELISA Kit, alpha-catenin ELISA Kit, alpha-ctn ELISA Kit, alphaCAT ELISA Kit, alphaCat ELISA Kit, alphacat ELISA Kit, Alpha-Cat ELISA Kit, dgri_GLEANR_3829 ELISA Kit, DgriGH19548 ELISA Kit, GH19548 ELISA Kit, ctnna ELISA Kit, cap102 ELISA Kit, catna1 ELISA Kit, ctnna1 ELISA Kit, catenin alpha 1 ELISA Kit, catenin (cadherin associated protein), alpha 1 ELISA Kit, alpha Catenin ELISA Kit, catenin alpha-1 ELISA Kit, catenin (cadherin-associated protein), alpha 1 ELISA Kit, catenin alpha 1 S homeolog ELISA Kit, CTNNA1 ELISA Kit, Ctnna1 ELISA Kit, alpha-Cat ELISA Kit, Dgri\alpha-Cat ELISA Kit, Tsp_10127 ELISA Kit, ctnna1 ELISA Kit, ctnna1.S ELISA Kit

Hintergrund:

Gene Name: Catenin Alpha 1

Gene Aliases: CTNN-A1, aCat, CAP102, Cadherin Associated Protein Alpha 1, Alpha E-catenin, Renal carcinoma antigen NY-REN-13

Gen-ID: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location