CD40 Ligand ELISA Kit
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- Target Alle CD40 Ligand (CD40LG) ELISA Kits anzeigen
- CD40 Ligand (CD40LG)
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 40 Ligand (CD40L). No significant cross-reactivity or interference between Cluster Of Differentiation 40 Ligand (CD40L) and analogues was observed.
- Sensitivität
- 0.056 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 40 Ligand (CD40L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 40 Ligand (CD40L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 40 Ligand (CD40L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 40 Ligand (CD40L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 40 Ligand (CD40L) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 40 Ligand (CD40L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle CD40 Ligand (CD40LG) ELISA Kits anzeigen
- CD40 Ligand (CD40LG)
- Andere Bezeichnung
- Cluster Of Differentiation 40 Ligand (CD40LG Produkte)
- Synonyme
- CD154 ELISA Kit, cd40l ELISA Kit, CD40L ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, T-BAM ELISA Kit, TNFSF5 ELISA Kit, TRAP ELISA Kit, gp39 ELISA Kit, hCD40L ELISA Kit, CD40-L ELISA Kit, Cd40l ELISA Kit, Ly-62 ELISA Kit, Ly62 ELISA Kit, Tnfsf5 ELISA Kit, CD40 ligand ELISA Kit, TNF superfamily member 5 ELISA Kit, CD40LG ELISA Kit, cd40l ELISA Kit, Cd40lg ELISA Kit
- Hintergrund
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Gene Name: Cluster Of Differentiation 40 Ligand
Gene Aliases: CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule
- Gen-ID
- 84349
- UniProt
- Q9Z2V2
- Pathways
- NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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