COL6A1 ELISA Kit
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- Target Alle COL6A1 ELISA Kits anzeigen
- COL6A1 (Collagen, Type VI, alpha 1 (COL6A1))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 62.5 pg/mL - 4000 pg/mL
- Untere Nachweisgrenze
- 62.5 pg/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Collagen Type VI Alpha 1 (COL6a1). No significant cross-reactivity or interference between Collagen Type VI Alpha 1 (COL6a1) and analogues was observed.
- Sensitivität
- 23.5 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type VI Alpha 1 (COL6a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type VI Alpha 1 (COL6a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type VI Alpha 1 (COL6a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type VI Alpha 1 (COL6a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type VI Alpha 1 (COL6a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type VI Alpha 1 (COL6a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle COL6A1 ELISA Kits anzeigen
- COL6A1 (Collagen, Type VI, alpha 1 (COL6A1))
- Andere Bezeichnung
- Collagen Type VI Alpha 1 (COL6A1 Produkte)
- Synonyme
- CBR ELISA Kit, SDR21C1 ELISA Kit, hCBR1 ELISA Kit, OPLL ELISA Kit, AI747156 ELISA Kit, Col6a-1 ELISA Kit, RGD1565398 ELISA Kit, COL6A1 ELISA Kit, carbonyl reductase 1 ELISA Kit, collagen type VI alpha 1 chain ELISA Kit, collagen, type VI, alpha 1 ELISA Kit, collagen, type VI, alpha 1 L homeolog ELISA Kit, collagen type VI alpha 3 chain ELISA Kit, collagen alpha-1(VI) chain ELISA Kit, CBR1 ELISA Kit, COL6A1 ELISA Kit, Col6a1 ELISA Kit, col6a1.L ELISA Kit, COL6A3 ELISA Kit, col6a1 ELISA Kit, LOC100623720 ELISA Kit
- Hintergrund
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Gene Name: Collagen Type VI Alpha 1
Gene Aliases: COL6-A1, OPLL, Collagen Alpha-1(VI)chain
- Gen-ID
- 1291
- UniProt
- P12109
- Pathways
- Growth Factor Binding, SARS-CoV-2 Protein Interaktom
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