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Complement C3 Convertase CLIA Kit

Reaktivität: Human Chemiluminescent Sandwich ELISA 0.39 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5653197
  • Target Alle Complement C3 Convertase Produkte
    Complement C3 Convertase
    Reaktivität
    • 4
    • 1
    • 1
    Human
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.39 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    0.39 ng/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Complement C3 Convertase (C3c). No significant cross-reactivity or interference between Complement C3 Convertase (C3c) and analogues was observed.
    Sensitivität
    0.06 ng/mL
    Featured
    Zu unserem meistverkauften Complement C3 Convertase ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 - 3 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with an antibody specific to Complement C3 Convertase (C3c). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement C3 Convertase (C3c). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement C3 Convertase (C3c) level in the sample or standard.,
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement C3 Convertase (C3c) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement C3 Convertase (C3c) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Complement C3 Convertase Produkte
    Complement C3 Convertase
    Abstract
    Complement C3 Convertase Produkte
    Hintergrund

    Gene Name: Complement C3 Convertase

    Gene Aliases: C4b2a

    Gen-ID
    718
    UniProt
    P01024
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