CD35 ELISA Kit

Produktnummer ABIN5653283

Produktdetails CD35 ELISA Kit

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Complement Receptor 1, Erythrocyte (CR1). No significant cross-reactivity or interference between Complement Receptor 1, Erythrocyte (CR1) and analogues was observed.

Sensitivität: 12.9 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Receptor 1, Erythrocyte (CR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Receptor 1, Erythrocyte (CR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Receptor 1, Erythrocyte (CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Receptor 1, Erythrocyte (CR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Andere Bezeichnung: Complement Receptor 1, Erythrocyte (CR1 Produkte)

Synonyme: C3BR ELISA Kit, C4BR ELISA Kit, CD35 ELISA Kit, KN ELISA Kit, C3DR ELISA Kit, CD21 ELISA Kit, Cr-1 ELISA Kit, Cr-2 ELISA Kit, Cr1 ELISA Kit, CR1-L ELISA Kit, CR2 ELISA Kit, CR1 ELISA Kit, complement C3b/C4b receptor 1 (Knops blood group) ELISA Kit, complement receptor 2 ELISA Kit, complement component (3d/Epstein Barr virus) receptor 2 ELISA Kit, complement receptor type 1 ELISA Kit, CR1 ELISA Kit, Cr2 ELISA Kit, CR2 ELISA Kit, LOC718120 ELISA Kit

Hintergrund:

Gene Name: Complement Receptor 1, Erythrocyte

Gene Aliases: CD35, C3BR, KN, C3b/C4b Receptor,Including Knops Blood Group System, Immune Adherence Receptor

Gen-ID: 1378

UniProt: P17927

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity