DOT1L ELISA Kit

Produktnummer ABIN5653731

Produktdetails DOT1L ELISA Kit

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of DOT1 Like, Histone H3 Methyltransferase (DOT1L). No significant cross-reactivity or interference between DOT1 Like, Histone H3 Methyltransferase (DOT1L) and analogues was observed.

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DOT1 Like, Histone H3 Methyltransferase (DOT1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DOT1 Like, Histone H3 Methyltransferase (DOT1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Andere Bezeichnung: DOT1 Like, Histone H3 Methyltransferase (DOT1L Produkte)

Synonyme: NV11307 ELISA Kit, DOT1L ELISA Kit, DOT1 ELISA Kit, KMT4 ELISA Kit, A630076O07 ELISA Kit, AW907654 ELISA Kit, Dot1 ELISA Kit, mDot1 ELISA Kit, histone-lysine N-methyltransferase, H3 lysine-79 specific ELISA Kit, DOT1 like histone lysine methyltransferase ELISA Kit, histone methyltransferase ELISA Kit, DOT1-like, histone H3 methyltransferase (S. cerevisiae) ELISA Kit, LOC100116648 ELISA Kit, DOT1L ELISA Kit, DOT1 ELISA Kit, Dot1l ELISA Kit

Hintergrund:

Gene Name: DOT1 Like, Histone H3 Methyltransferase

Gene Aliases: DOT1, KMT4, Histone-lysine N-methyltransferase, H3 lysine-79 specific, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4