DYNC1H1 ELISA Kit

Produktnummer ABIN5653757

Produktdetails DYNC1H1 ELISA Kit

Target: DYNC1H1 (Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). No significant cross-reactivity or interference between Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) and analogues was observed.

Sensitivität: 0.113 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu DYNC1H1

Target: DYNC1H1 (Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1))

Andere Bezeichnung: Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1 Produkte)

Synonyme: fk70a07 ELISA Kit, wu:fk70a07 ELISA Kit, p22 ELISA Kit, dhc1 ELISA Kit, dncl ELISA Kit, dyhc ELISA Kit, hl-3 ELISA Kit, dhc1a ELISA Kit, dnch1 ELISA Kit, dnecl ELISA Kit, dnchc1 ELISA Kit, Dnch1 ELISA Kit, Dnchc1 ELISA Kit, 9930018I23Rik ELISA Kit, AI894280 ELISA Kit, DHC1 ELISA Kit, DHC1a ELISA Kit, DNCL ELISA Kit, Dnec1 ELISA Kit, Dnecl ELISA Kit, Loa ELISA Kit, MAP1C ELISA Kit, P22 ELISA Kit, Swl ELISA Kit, mKIAA0325 ELISA Kit, DNCH1 ELISA Kit, DNECL ELISA Kit, DYHC ELISA Kit, HL-3 ELISA Kit, SMALED1 ELISA Kit, dynein, cytoplasmic 1, heavy chain 1 ELISA Kit, dynein cytoplasmic 1 heavy chain 1 ELISA Kit, dync1h1 ELISA Kit, DYNC1H1 ELISA Kit, Dync1h1 ELISA Kit

Hintergrund:

Gene Name: Dynein, Cytoplasmic 1, Heavy Chain 1

Gene Aliases: DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, p22, Dynein heavy chain, cytosolic, Cytoplasmic dynein heavy chain 1, Cytoplasmic dynein 1 heavy chain 1

Gen-ID: 1778

UniProt: Q14204

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization