ENPP2 ELISA Kit

Produktnummer ABIN5653782

Produktdetails ENPP2 ELISA Kit

Target: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2). No significant cross-reactivity or interference between Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) and analogues was observed.

Sensitivität: 0.065 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ENPP2

Target: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Andere Bezeichnung: Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2 Produkte)

Synonyme: ATX ELISA Kit, ATX-X ELISA Kit, AUTOTAXIN ELISA Kit, LysoPLD ELISA Kit, NPP2 ELISA Kit, PD-IALPHA ELISA Kit, PDNP2 ELISA Kit, Npps2 ELISA Kit, PD-Ialpha ELISA Kit, Pdnp2 ELISA Kit, MGC132047 ELISA Kit, atx ELISA Kit, zgc:63550 ELISA Kit, autotaxin ELISA Kit, enpp2 ELISA Kit, enpp2a ELISA Kit, enpp2b ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 L homeolog ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 S homeolog ELISA Kit, ENPP2 ELISA Kit, Enpp2 ELISA Kit, enpp2 ELISA Kit, enpp2.L ELISA Kit, enpp2.S ELISA Kit

Hintergrund:

Gene Name: Ectonucleotide Pyrophosphatase/Phosphodiesterase 2

Gene Aliases: ATX, ATX-X, LysoPLD, NPP2, PD-IALPHA, PDNP2, Autotaxin, Extracellular lysophospholipase D

Gen-ID: 84050

UniProt: Q64610