CELA2A ELISA Kit

Produktnummer ABIN5653797

Produktdetails CELA2A ELISA Kit

Target: CELA2A (Chymotrypsin-Like Elastase Family, Member 2A (CELA2A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Elastase 2A (ELA2A). No significant cross-reactivity or interference between Elastase 2A (ELA2A) and analogues was observed.

Sensitivität: 22.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 2A (ELA2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 2A (ELA2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 2A (ELA2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 2A (ELA2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 2A (ELA2A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 2A (ELA2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CELA2A

Target: CELA2A (Chymotrypsin-Like Elastase Family, Member 2A (CELA2A))

Andere Bezeichnung: Elastase 2A (CELA2A Produkte)

Synonyme: Ela-2 ELISA Kit, Ela2 ELISA Kit, Ela2a ELISA Kit, ELAII ELISA Kit, Elaii1 ELISA Kit, ELA2A ELISA Kit, PE-1 ELISA Kit, ela2 ELISA Kit, ela2a ELISA Kit, ELA2 ELISA Kit, ELANE ELISA Kit, PE2 ELISA Kit, cela2a ELISA Kit, chymotrypsin-like elastase family, member 2A ELISA Kit, chymotrypsin like elastase family member 2A ELISA Kit, chymotrypsin-like elastase family member 2A ELISA Kit, elastase-2A ELISA Kit, elastase 2A ELISA Kit, chymotrypsin like elastase family member 2A L homeolog ELISA Kit, Cela2a ELISA Kit, CELA2A ELISA Kit, cela2a ELISA Kit, LOC711204 ELISA Kit, CpipJ_CPIJ003622 ELISA Kit, CpipJ_CPIJ003629 ELISA Kit, CpipJ_CPIJ003630 ELISA Kit, CpipJ_CPIJ010791 ELISA Kit, Tsp_10977 ELISA Kit, LOC100227420 ELISA Kit, cela2a.L ELISA Kit

Hintergrund:

Gene Name: Elastase 2A

Gene Aliases: ELA1, PE-1, CELA2A, Chymotrypsin-Like Elastase Family Member 2A

Gen-ID: 24332

UniProt: P00774