ECE1 ELISA Kit

Produktnummer ABIN5653866

Produktdetails ECE1 ELISA Kit

Target: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Endothelin Converting Enzyme 1 (ECE1). No significant cross-reactivity or interference between Endothelin Converting Enzyme 1 (ECE1) and analogues was observed.

Sensitivität: 11.8 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin Converting Enzyme 1 (ECE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelin Converting Enzyme 1 (ECE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelin Converting Enzyme 1 (ECE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelin Converting Enzyme 1 (ECE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin Converting Enzyme 1 (ECE1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin Converting Enzyme 1 (ECE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ECE1

Target: ECE1 (Endothelin-Converting Enzyme 1 (ECE1))

Andere Bezeichnung: Endothelin Converting Enzyme 1 (ECE1 Produkte)

Synonyme: ECE ELISA Kit, Ece ELISA Kit, AW322500 ELISA Kit, ECE-1 ELISA Kit, ECE-1a ELISA Kit, ECE-1b ELISA Kit, ECE-1d ELISA Kit, ece1 ELISA Kit, MGC52874 ELISA Kit, MGC130791 ELISA Kit, fd11b07 ELISA Kit, wu:fd11b07 ELISA Kit, zgc:154079 ELISA Kit, endothelin converting enzyme 1 ELISA Kit, endothelin converting enzyme like 1 ELISA Kit, endothelin converting enzyme 1 L homeolog ELISA Kit, endothelin-converting enzyme 1 ELISA Kit, Endothelin-converting enzyme 1 ELISA Kit, ECE1 ELISA Kit, ECEL1 ELISA Kit, Ece1 ELISA Kit, ece1.L ELISA Kit, Za10_0551 ELISA Kit, Arch_0474 ELISA Kit, Closa_0961 ELISA Kit, Odosp_0578 ELISA Kit, Bacsa_1787 ELISA Kit, Weevi_2017 ELISA Kit, Fluta_3444 ELISA Kit, Sphch_2390 ELISA Kit, ece1 ELISA Kit

Hintergrund:

Gene Name: Endothelin Converting Enzyme 1

Gen-ID: 1889

UniProt: P42892

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling