EZH2 CLIA Kit

Produktnummer ABIN5653875

Produktdetails EZH2 CLIA Kit

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Enhancer Of Zeste Homolog 2 (EZH2). No significant cross-reactivity or interference between Enhancer Of Zeste Homolog 2 (EZH2) and analogues was observed.

Sensitivität: 5.69 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Enhancer Of Zeste Homolog 2 (EZH2) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enhancer Of Zeste Homolog 2 (EZH2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enhancer Of Zeste Homolog 2 (EZH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Andere Bezeichnung: Enhancer Of Zeste Homolog 2 (EZH2 Produkte)

Synonyme: ENX-1 CLIA Kit, ENX1 CLIA Kit, EZH1 CLIA Kit, EZH2b CLIA Kit, KMT6 CLIA Kit, KMT6A CLIA Kit, WVS CLIA Kit, WVS2 CLIA Kit, eya2 CLIA Kit, im:7149338 CLIA Kit, zgc:136376 CLIA Kit, zgc:152758 CLIA Kit, EZH2 CLIA Kit, enx-1 CLIA Kit, enx1 CLIA Kit, ezh1 CLIA Kit, kmt6 CLIA Kit, xez CLIA Kit, Enx-1 CLIA Kit, Enx1h CLIA Kit, mKIAA4065 CLIA Kit, ezh2 CLIA Kit, olezh2 CLIA Kit, enhancer of zeste 2 polycomb repressive complex 2 subunit CLIA Kit, enhancer of zeste 2 polycomb repressive complex 2 subunit L homeolog CLIA Kit, enhancer of zeste 2 polycomb repressive complex 2 subunit S homeolog CLIA Kit, EZH2 CLIA Kit, ezh2 CLIA Kit, Ezh2 CLIA Kit, ezh2.L CLIA Kit, ezh2.S CLIA Kit

Hintergrund:

Gene Name: Enhancer Of Zeste Homolog 2

Gene Aliases: ENX-1, KMT6, KMT6A, Histone-lysine N-methyltransferase EZH2, Lysine N-methyltransferase 6

Gen-ID: 2146

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation