EPH Receptor A3 ELISA Kit

Produktnummer ABIN5653917

Produktdetails EPH Receptor A3 ELISA Kit

Target: EPH Receptor A3 (EPHA3)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ephrin Type A Receptor 3 (EPHA3). No significant cross-reactivity or interference between Ephrin Type A Receptor 3 (EPHA3) and analogues was observed.

Sensitivität: 0.113 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type A Receptor 3 (EPHA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin Type A Receptor 3 (EPHA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type A Receptor 3 (EPHA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type A Receptor 3 (EPHA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin Type A Receptor 3 (EPHA3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin Type A Receptor 3 (EPHA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu EPH Receptor A3

Target: EPH Receptor A3 (EPHA3)

Andere Bezeichnung: Ephrin Type A Receptor 3 (EPHA3 Produkte)

Synonyme: EK4 ELISA Kit, ETK ELISA Kit, ETK1 ELISA Kit, HEK ELISA Kit, HEK4 ELISA Kit, TYRO4 ELISA Kit, EPHA3 ELISA Kit, AW492086 ELISA Kit, Cek4 ELISA Kit, End3 ELISA Kit, Hek ELISA Kit, Hek4 ELISA Kit, Mek4 ELISA Kit, Tyro4 ELISA Kit, CEK4 ELISA Kit, EPH receptor A3 ELISA Kit, ephrin type-A receptor 3 ELISA Kit, Eph receptor A3 ELISA Kit, EPHA3 ELISA Kit, LOC100439679 ELISA Kit, Epha3 ELISA Kit

Hintergrund:

Gene Name: Ephrin Type A Receptor 3

Gene Aliases: ETK, ETK1, HEK, HEK4, TYRO4, Human embryo kinase, Tyrosine-protein kinase receptor ETK1

Pathways: RTK Signalweg, Regulation of Cell Size