ESRRA ELISA Kit

Produktnummer ABIN5653994

Produktdetails ESRRA ELISA Kit

Target: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Estrogen Related Receptor Alpha (ERRa). No significant cross-reactivity or interference between Estrogen Related Receptor Alpha (ERRa) and analogues was observed.

Sensitivität: 0.127 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Related Receptor Alpha (ERRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Related Receptor Alpha (ERRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Related Receptor Alpha (ERRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Related Receptor Alpha (ERRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Related Receptor Alpha (ERRa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Related Receptor Alpha (ERRa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ESRRA

Target: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Andere Bezeichnung: Estrogen Related Receptor Alpha (ESRRA Produkte)

Synonyme: err1 ELISA Kit, erra ELISA Kit, esrral ELISA Kit, erralpha ELISA Kit, nr3b1 ELISA Kit, MGC80854 ELISA Kit, ESRRA ELISA Kit, esrl1 ELISA Kit, LOC100125507 ELISA Kit, ERRalpha ELISA Kit, Err1 ELISA Kit, Estrra ELISA Kit, Nr3b1 ELISA Kit, Errra ELISA Kit, ERR1 ELISA Kit, ERRa ELISA Kit, ESRL1 ELISA Kit, NR3B1 ELISA Kit, ESTRRA ELISA Kit, estrogen-related receptor alpha ELISA Kit, estrogen related receptor alpha L homeolog ELISA Kit, estrogen related receptor alpha ELISA Kit, catsper channel auxiliary subunit zeta ELISA Kit, estrogen related receptor, alpha ELISA Kit, esrra ELISA Kit, esrra.L ELISA Kit, ESRRA ELISA Kit, LOC100125507 ELISA Kit, CATSPERZ ELISA Kit, Esrra ELISA Kit

Hintergrund:

Gene Name: Estrogen Related Receptor Alpha

Gene Aliases: ERR1, ERRa, ERRalpha, ESRL1, NR3B1, Estrogen receptor-like 1, Nuclear receptor subfamily 3 group B member 1

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha