PERK ELISA Kit

Produktnummer ABIN5654005

Produktdetails PERK ELISA Kit

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) and analogues was observed.

Sensitivität: 0.108 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PERK

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Andere Bezeichnung: Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2AK3 Produkte)

Synonyme: EIF2AK3 ELISA Kit, AI427929 ELISA Kit, Pek ELISA Kit, Perk ELISA Kit, PEK ELISA Kit, PERK ELISA Kit, WRS ELISA Kit, eukaryotic translation initiation factor 2 alpha kinase 3 ELISA Kit, EIF2AK3 ELISA Kit, Eif2ak3 ELISA Kit

Hintergrund:

Gene Name: Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3

Gene Aliases: WRS, PEK, PERK, HsPEK, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase

Gen-ID: 13666

UniProt: Q9Z2B5

Pathways: Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response