FBXL3 ELISA Kit

Produktnummer ABIN5654031

Produktdetails FBXL3 ELISA Kit

Target: FBXL3 (F-Box and Leucine-Rich Repeat Protein 3 (FBXL3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of F-Box And Leucine Rich Repeat Protein 3 (FBXL3). No significant cross-reactivity or interference between F-Box And Leucine Rich Repeat Protein 3 (FBXL3) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to F-Box And Leucine Rich Repeat Protein 3 (FBXL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to F-Box And Leucine Rich Repeat Protein 3 (FBXL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F-Box And Leucine Rich Repeat Protein 3 (FBXL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-Box And Leucine Rich Repeat Protein 3 (FBXL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F-Box And Leucine Rich Repeat Protein 3 (FBXL3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F-Box And Leucine Rich Repeat Protein 3 (FBXL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FBXL3

Target: FBXL3 (F-Box and Leucine-Rich Repeat Protein 3 (FBXL3))

Andere Bezeichnung: F-Box And Leucine Rich Repeat Protein 3 (FBXL3 Produkte)

Synonyme: FBL3 ELISA Kit, FBL3A ELISA Kit, FBXL3A ELISA Kit, AU041772 ELISA Kit, AW212966 ELISA Kit, FBK ELISA Kit, Fbl3a ELISA Kit, Fbxl3a ELISA Kit, Ovtm ELISA Kit, Play68 ELISA Kit, fbxl3 ELISA Kit, FBXL3 ELISA Kit, F7A7.240 ELISA Kit, F7A7_240 ELISA Kit, im:7153024 ELISA Kit, zgc:103721 ELISA Kit, F-box and leucine rich repeat protein 3 ELISA Kit, F-box and leucine-rich repeat protein 3 ELISA Kit, F-box and leucine-rich repeat protein 3 L homeolog ELISA Kit, RNI-like superfamily protein ELISA Kit, F-box and leucine-rich repeat protein 3a ELISA Kit, FBXL3 ELISA Kit, Fbxl3 ELISA Kit, fbxl3.L ELISA Kit, fbxl3 ELISA Kit, AT5G01720 ELISA Kit, fbxl3a ELISA Kit

Hintergrund:

Gene Name: F-Box And Leucine Rich Repeat Protein 3

Gene Aliases: FBL3, FBL3A, FBXL3A, F-box/LRR-repeat protein 3A

Gen-ID: 50789

UniProt: Q8C4V4

Pathways: Photoperiodism