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FAM3A ELISA Kit

FAM3A Reaktivität: Human Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5654047
  • Target Alle FAM3A ELISA Kits anzeigen
    FAM3A (Family with Sequence Similarity 3, Member A (FAM3A))
    Reaktivität
    • 4
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.62 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    15.62 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Family With Sequence Similarity 3, Member A (FAM3A). No significant cross-reactivity or interference between Family With Sequence Similarity 3, Member A (FAM3A) and analogues was observed.
    Sensitivität
    26.1 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 3, Member A (FAM3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 3, Member A (FAM3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 3, Member A (FAM3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Family With Sequence Similarity 3, Member A (FAM3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Family With Sequence Similarity 3, Member A (FAM3A) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Family With Sequence Similarity 3, Member A (FAM3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle FAM3A ELISA Kits anzeigen
    FAM3A (Family with Sequence Similarity 3, Member A (FAM3A))
    Andere Bezeichnung
    Family With Sequence Similarity 3, Member A (FAM3A Produkte)
    Synonyme
    MGC53212 ELISA Kit, MGC79808 ELISA Kit, 2.19 ELISA Kit, DLD ELISA Kit, DXS560S ELISA Kit, XAP-7 ELISA Kit, 1810037C20Rik ELISA Kit, RP23-436K3.15 ELISA Kit, RGD1562076 ELISA Kit, family with sequence similarity 3 member A L homeolog ELISA Kit, family with sequence similarity 3 member A ELISA Kit, family with sequence similarity 3, member A ELISA Kit, fam3a.L ELISA Kit, fam3a ELISA Kit, FAM3A ELISA Kit, Fam3a ELISA Kit
    Hintergrund

    Gene Name: Family With Sequence Similarity 3, Member A

    Gene Aliases: XAP-7, Cytokine-like protein 2-19

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