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FGF13 CLIA Kit

FGF13 Reaktivität: Human Chemiluminescent Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5654205
  • Target Alle FGF13 CLIA Kits anzeigen
    FGF13 (Fibroblast Growth Factor 13 (FGF13))
    Reaktivität
    • 6
    • 3
    • 3
    Human
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Competition ELISA
    Detektionsbereich
    6.17 pg/mL - 500 pg/mL
    Untere Nachweisgrenze
    6.17 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 13 (FGF13). No significant cross-reactivity or interference between Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
    Sensitivität
    2.42 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Fibroblast Growth Factor 13 (FGF13). A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 13 (FGF13) and unlabeled Fibroblast Growth Factor 13 (FGF13) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 13 (FGF13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 13 (FGF13) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Fibroblast Growth Factor 13 (FGF13) level in the sample or standard.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle FGF13 CLIA Kits anzeigen
    FGF13 (Fibroblast Growth Factor 13 (FGF13))
    Andere Bezeichnung
    Fibroblast Growth Factor 13 (FGF13 Produkte)
    Synonyme
    FGF13 CLIA Kit, fgf2 CLIA Kit, fhf2 CLIA Kit, fgf13 CLIA Kit, FGF-13 CLIA Kit, xFGF13 CLIA Kit, FGF2 CLIA Kit, FHF-2 CLIA Kit, FHF2 CLIA Kit, Fhf2 CLIA Kit, zgc:101784 CLIA Kit, fibroblast growth factor 13 CLIA Kit, fibroblast growth factor 13 L homeolog CLIA Kit, fibroblast growth factor 13a CLIA Kit, FGF13 CLIA Kit, fgf13 CLIA Kit, fgf13.L CLIA Kit, Fgf13 CLIA Kit, fgf13a CLIA Kit
    Hintergrund

    Gene Name: Fibroblast Growth Factor 13

    Gene Aliases: FHF2, Fibroblast Growth Factor Homologous Factor 2

    Gen-ID
    2258
    UniProt
    Q92913
    Pathways
    Regulation of Cell Size
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