FGF4 ELISA Kit

Produktnummer ABIN5654264

Produktdetails FGF4 ELISA Kit

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 4 (FGF4). No significant cross-reactivity or interference between Fibroblast Growth Factor 4 (FGF4) and analogues was observed.

Sensitivität: 5.7 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 4 (FGF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 4 (FGF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 4 (FGF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 4 (FGF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 4 (FGF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FGF4

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Andere Bezeichnung: Fibroblast Growth Factor 4 (FGF4 Produkte)

Synonyme: HBGF-4 ELISA Kit, HST ELISA Kit, HST-1 ELISA Kit, HSTF1 ELISA Kit, K-FGF ELISA Kit, KFGF ELISA Kit, Fgf-4 ELISA Kit, Fgfk ELISA Kit, Hst1 ELISA Kit, Hstf-1 ELISA Kit, KS3 ELISA Kit, hst ELISA Kit, hst-1 ELISA Kit, kFGF ELISA Kit, Hst ELISA Kit, fibroblast growth factor 4 ELISA Kit, FGF4 ELISA Kit, Fgf4 ELISA Kit

Hintergrund:

Gene Name: Fibroblast Growth Factor 4

Gene Aliases: HBGF4, HST, HSTF1, KFGF, K-FGF, Heparin Secretory Transforming Protein 1, Kaposi Sarcoma Oncogene, Transforming Protein KS3, FGF-Related Oncogene

UniProt: P48803

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance