MTOR ELISA Kit
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- Target Alle MTOR (mTOR) ELISA Kits anzeigen
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP). No significant cross-reactivity or interference between FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) and analogues was observed.
- Sensitivität
- 0.114 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle MTOR (mTOR) ELISA Kits anzeigen
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- Andere Bezeichnung
- FK506 Binding Protein 12 Rapamycin Associated Protein (mTOR Produkte)
- Synonyme
- FRAP1 ELISA Kit, FRAP ELISA Kit, FRAP2 ELISA Kit, RAFT1 ELISA Kit, RAPT1 ELISA Kit, 2610315D21Rik ELISA Kit, AI327068 ELISA Kit, Frap1 ELISA Kit, flat ELISA Kit, frap1 ELISA Kit, tor ELISA Kit, wu:fc22h08 ELISA Kit, mechanistic target of rapamycin kinase ELISA Kit, mechanistic target of rapamycin ELISA Kit, mechanistic target of rapamycin (serine/threonine kinase) ELISA Kit, MTOR ELISA Kit, Mtor ELISA Kit, mtor ELISA Kit
- Hintergrund
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Gene Name: FK506 Binding Protein 12 Rapamycin Associated Protein
Gene Aliases: FRAP1, MTOR, FRAP2, MTOR, RAFT1, RAPT1, Mammalian Target Of Rapamycin, FKBP12-Rapamycin Complex-Associated Protein 1, Rapamycin and FKBP12 target 1
- Gen-ID
- 2475
- UniProt
- P42345
- Pathways
- PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effekt
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