FKBP1B ELISA Kit

Produktnummer ABIN5654358

Produktdetails FKBP1B ELISA Kit

Target: FKBP1B (FK506 Binding Protein 1B, 12.6 KDa (FKBP1B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 1B (FKBP1B). No significant cross-reactivity or interference between FK506 Binding Protein 1B (FKBP1B) and analogues was observed.

Sensitivität: 0.053 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1B (FKBP1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 1B (FKBP1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 1B (FKBP1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 1B (FKBP1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FKBP1B

Target: FKBP1B (FK506 Binding Protein 1B, 12.6 KDa (FKBP1B))

Andere Bezeichnung: FK506 Binding Protein 1B (FKBP1B Produkte)

Synonyme: FKBP12.6 ELISA Kit, MGC68829 ELISA Kit, FKBP1B ELISA Kit, DKFZp459K1429 ELISA Kit, 12.6kDa ELISA Kit, AW494148 ELISA Kit, calstabin2 ELISA Kit, FKBP1L ELISA Kit, OTK4 ELISA Kit, PKBP1L ELISA Kit, PPIase ELISA Kit, FK506 binding protein 1B ELISA Kit, FK506 binding protein 1B L homeolog ELISA Kit, FK506 binding protein 1b ELISA Kit, FKBP1B ELISA Kit, fkbp1b.L ELISA Kit, fkbp1b ELISA Kit, Fkbp1b ELISA Kit

Hintergrund:

Gene Name: FK506 Binding Protein 1B

Gene Aliases: FKBP12.6, FKBP1L, FKBP9, OTK4, PKBP1L, Rotamase, 12.6 kDa FK506-binding protein, Immunophilin FKBP12.6, Peptidyl-prolyl cis-trans isomerase FKBP1B

Gen-ID: 2281

UniProt: P68106

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Negative Regulation of Transporter Activity