FUCA2 ELISA Kit

Produktnummer ABIN5654438

Produktdetails FUCA2 ELISA Kit

Target: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Fucosidase Alpha L2, Plasma (FUCa2). No significant cross-reactivity or interference between Fucosidase Alpha L2, Plasma (FUCa2) and analogues was observed.

Sensitivität: 6.8 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fucosidase Alpha L2, Plasma (FUCa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fucosidase Alpha L2, Plasma (FUCa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fucosidase Alpha L2, Plasma (FUCa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fucosidase Alpha L2, Plasma (FUCa2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fucosidase Alpha L2, Plasma (FUCa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FUCA2

Target: FUCA2 (Fucosidase, alpha-L- 2, Plasma (FUCA2))

Andere Bezeichnung: Fucosidase Alpha L2, Plasma (FUCA2 Produkte)

Synonyme: MGC68702 ELISA Kit, zgc:92013 ELISA Kit, wu:fc20c06 ELISA Kit, F10M10.30 ELISA Kit, F10M10_30 ELISA Kit, SMb20490 ELISA Kit, dJ20N2.5 ELISA Kit, 0610025O11Rik ELISA Kit, 5530401P20Rik ELISA Kit, alpha-L-fucosidase 2 L homeolog ELISA Kit, fucosidase, alpha-L- 2, plasma ELISA Kit, alpha-L-fucosidase 2 ELISA Kit, 1,2-alpha-L-fucosidase ELISA Kit, methylthioribulose-1-phosphate dehydratase ELISA Kit, fuculose-1-phosphate aldolase ELISA Kit, L-fuculose phosphate aldolase ELISA Kit, fuca2.L ELISA Kit, FUCA2 ELISA Kit, fuca2 ELISA Kit, FUC95A ELISA Kit, LOC100282758 ELISA Kit, LOC100284864 ELISA Kit, LOC100284892 ELISA Kit, LOC100285541 ELISA Kit, LOC100285863 ELISA Kit, LOC100286039 ELISA Kit, fucA2 ELISA Kit, Fuca2 ELISA Kit

Hintergrund:

Gene Name: Fucosidase Alpha L2, Plasma

Gene Aliases: Alpha-L-fucoside fucohydrolase 2

Gen-ID: 2519

UniProt: Q9BTY2