FAH ELISA Kit

Produktnummer ABIN5654443

Produktdetails FAH ELISA Kit

Target: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Fumarylacetoacetate Hydrolase (FAH). No significant cross-reactivity or interference between Fumarylacetoacetate Hydrolase (FAH) and analogues was observed.

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fumarylacetoacetate Hydrolase (FAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fumarylacetoacetate Hydrolase (FAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fumarylacetoacetate Hydrolase (FAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fumarylacetoacetate Hydrolase (FAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fumarylacetoacetate Hydrolase (FAH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fumarylacetoacetate Hydrolase (FAH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FAH

Target: FAH (Fumarylacetoacetate Hydrolase (Fumarylacetoacetase) (FAH))

Andere Bezeichnung: Fumarylacetoacetate Hydrolase (FAH Produkte)

Synonyme: CG14993 ELISA Kit, Dmel\\CG14993 ELISA Kit, dfaa ELISA Kit, l(3)64Al ELISA Kit, l(3)SH11 ELISA Kit, PSPTO3550 ELISA Kit, fb59b12 ELISA Kit, wu:fb59b12 ELISA Kit, zgc:55316 ELISA Kit, FAA ELISA Kit, Fumarylacetoacetase ELISA Kit, FumarylAcetoacetate Hydrolase ELISA Kit, fumarylacetoacetase ELISA Kit, fumarylacetoacetate hydrolase ELISA Kit, fumarylacetoacetate hydrolase (fumarylacetoacetase) ELISA Kit, fumarylacetoacetate hydrolase (fumarylacetoacetase) L homeolog ELISA Kit, Faa ELISA Kit, fah-1 ELISA Kit, fahA ELISA Kit, hmgB ELISA Kit, Ndas_1870 ELISA Kit, Lbys_3503 ELISA Kit, Riean_0663 ELISA Kit, Ftrac_0820 ELISA Kit, Celal_3675 ELISA Kit, Celly_2655 ELISA Kit, Weevi_0208 ELISA Kit, Fluta_2435 ELISA Kit, Halhy_2019 ELISA Kit, Lacal_2931 ELISA Kit, FsymDg_1892 ELISA Kit, Fah ELISA Kit, fah ELISA Kit, fah.L ELISA Kit, FAH ELISA Kit

Hintergrund:

Gene Name: Fumarylacetoacetate Hydrolase

Gene Aliases: FAA, Fumarylacetoacetase, Tyrosinemia 1, Beta-diketonase

Gen-ID: 2184

UniProt: P16930