FYN ELISA Kit

Produktnummer ABIN5654449

Produktdetails FYN ELISA Kit

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of FYN Oncogene Related To SRC/FGR/YES (FYN). No significant cross-reactivity or interference between FYN Oncogene Related To SRC/FGR/YES (FYN) and analogues was observed.

Sensitivität: 0.06 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FYN Oncogene Related To SRC/FGR/YES (FYN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FYN Oncogene Related To SRC/FGR/YES (FYN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FYN Oncogene Related To SRC/FGR/YES (FYN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FYN Oncogene Related To SRC/FGR/YES (FYN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FYN Oncogene Related To SRC/FGR/YES (FYN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Andere Bezeichnung: FYN Oncogene Related To SRC/FGR/YES (FYN Produkte)

Synonyme: im:6908851 ELISA Kit, si:dkey-119m7.5 ELISA Kit, zgc:109996 ELISA Kit, c-fyn ELISA Kit, cfyn-a ELISA Kit, SLK ELISA Kit, SYN ELISA Kit, p59-FYN ELISA Kit, PKC ELISA Kit, fyn ELISA Kit, zgc:86720 ELISA Kit, AI448320 ELISA Kit, AW552119 ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase S homeolog ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase b ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase L homeolog ELISA Kit, FYN proto-oncogene, Src family tyrosine kinase a ELISA Kit, Fyn proto-oncogene ELISA Kit, FYN ELISA Kit, fyn.S ELISA Kit, fynb ELISA Kit, fyn.L ELISA Kit, Fyn ELISA Kit, fyna ELISA Kit

Hintergrund:

Gene Name: FYN Oncogene Related To SRC/FGR/YES

Gene Aliases: SLK, SYN, c-Fyn, Src-like kinase

Gen-ID: 2534

UniProt: P06241

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling