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GPER ELISA Kit

GPER Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5654454
  • Target Alle GPER ELISA Kits anzeigen
    GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))
    Reaktivität
    • 4
    • 2
    • 2
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of G Protein Coupled Estrogen Receptor 1 (GPER). No significant cross-reactivity or interference between G Protein Coupled Estrogen Receptor 1 (GPER) and analogues was observed.
    Sensitivität
    0.125 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Estrogen Receptor 1 (GPER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Estrogen Receptor 1 (GPER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle GPER ELISA Kits anzeigen
    GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))
    Andere Bezeichnung
    G Protein Coupled Estrogen Receptor 1 (GPER Produkte)
    Synonyme
    CEPR ELISA Kit, CMKRL2 ELISA Kit, DRY12 ELISA Kit, FEG-1 ELISA Kit, GPCR-Br ELISA Kit, GPER ELISA Kit, GPR30 ELISA Kit, LERGU ELISA Kit, LERGU2 ELISA Kit, LyGPR ELISA Kit, 6330420K13Rik ELISA Kit, Ceprl ELISA Kit, Gper ELISA Kit, Gpr30 ELISA Kit, GPR41 ELISA Kit, GPCR30 ELISA Kit, G protein-coupled estrogen receptor 1 ELISA Kit, GPER1 ELISA Kit, GPER ELISA Kit, Gper1 ELISA Kit
    Hintergrund

    Gene Name: G Protein Coupled Estrogen Receptor 1

    Gene Aliases: GPR30, CMKRL2, DRY12, FEG-1, GPCR-Br, LERGU, LyGPR, Membrane estrogen receptor, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1

    Pathways
    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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